Size Exclusion / Ion Exchange Columns
FIAlab Size Exclusion and Ion Exchange Columns
- Sample volumes: 0.125 to 1.0 mL
- Bed volumes: 125 μL to 1.25 mL
- Resins: Sephadex G25, G100, Sepharose 4% & 6%
- Custom resin packing available
Benefits:
- Removes interfering substances
- Improves accuracy & reproducibility
- Enhances LC-MS/MS baseline stability
Applications:
- Sample cleanup before analysis
Optimize your sample cleanup with FIAlab's customizable columns.
FIAlab manufactures a range of Size Exclusion and Ion Exchange columns tailored to streamline sample cleanup procedures in laboratory settings. These versatile columns are designed to accommodate sample volumes ranging from 0.125 to 1.0 milliliter, making them suitable for various analytical applications.
| Part Number | Material Type | Description |
|---|---|---|
| 770047 | G25 Sephadex | Size exclusion column with 33-140um bead size and >8 x 10^3 exclusion limit (Mr). |
| 770048 | G100 Sephadex | Size exclusion column with 49-134um bead size and >1 x 10^5 exclusion limit (Mr). |
| 770049 | Sepharose 4% | Size exclusion column with 45-165um bead size and >2 x 10^7 exclusion limit (Mr). |
| 770050 | Sepharose 6% | Size exclusion column with ~90um bead size and ~4 x 10^6 exclusion limit (Mr). |
The bed volumes of these columns vary from 125 microliters to 1.25 milliliters, providing researchers with options to match their specific sample cleanup requirements. Resins available for these columns include Sephadex G25, G100, and Sepharose 4% and 6%. Additionally, FIAlab offers custom resin packing services upon request, allowing researchers to tailor the columns to their unique analytical needs.
Size Exclusion and Ion Exchange columns find frequent use in cleaning up a sample's matrix before analysis, particularly in applications where removal of interfering substances is essential for accurate results. Sephadex G25 and G100 resins are commonly employed to eliminate salts from peptide maps before trypsin digestion. The presence of salts in samples can lead to baseline instability in tandem Liquid Chromatogram - Mass Spectrometer (LC-MS/MS) systems, affecting the accuracy and reliability of analytical data. By employing Size Exclusion and Ion Exchange columns, researchers can effectively remove these interfering substances, enhancing the quality and reproducibility of their analytical results.