Pharmaceutical
FIAlab’s ProSIAmpler uses a liquid handling technique that automates important processes like sterile sampling, liquid handling, and measurement; this system is the leading bioreactor autosampler on the market. When combined with the SIAlock, 250CC, and SIAsoft, this system offers a multitude of benefits for laboratory operations. The ProSIAmpler, with its variable sample volume, draws ranging from 25uL to 10,000uL, providing flexibility and precision in sample handling. It can efficiently draw and analyze bioreactor samples, saving time and increasing automation.
The ProSIAmpler can monitor bioreactors' nutrients, pH, cell densities, and viabilities, in-situ, using ion-selective electrodes (ISE). This unlocks the potentiometric determination of several bioreactor parameters that are essential for optimal conditions where the ProSIAmpler can be programmed to automatically sample upon certain analyte concentrations or pH levels.
A sterile barrier is maintained with the SIAlock. The SIAlock is designed as single-use and eliminates the need for disassembly and cleaning, streamlining the workflow and reducing the risk of cross-contamination. Its liquid-based sample transport system, which is more robust than air-based systems, ensures reliable and accurate sample transfer. With an incredibly low internal volume of approximately 185uL, the SIAlock minimizes sample waste and enhances the overall efficiency of the system, making it suitable for processing thousands of samples before replacement.
The integration of the FIAlab 250uL Clarification Cartridge (250CC) into the ProSIAmpler system further enhances its capabilities. The 250CC cartridge offers exceptional clarification performance, capable of clarifying samples down to 25uL, which is 40 times lower than most competitors. Its compact design allows for seamless incorporation into the ProSIAmpler, optimizing the use of laboratory space. Real-time monitoring of the 250CC cartridge's efficiency through SIAsoft provides valuable insights into the clarification process, ensuring optimal performance and quality control. The 250CC cartridge is designed for long-term use, with the ability to be utilized up to 1,000 times, reducing operational costs and waste. It has demonstrated successful clarification of cells up to 50x10^6 cell/mL, making it suitable for a wide range of sample concentrations. Additionally, the external cell removal feature of the 250CC cartridge enables both multi-reactor clarification and quick replacement, minimizing downtime and ensuring continuous workflow.
The ProSIAmpler system is powered by FIAlab's in-house SIAsoft software, which offers seamless integration of the autosampler, liquid handler, and detection modules. This integration streamlines the entire workflow, allowing for efficient and automated sample handling. The SIAsoft software, with its flexible AI feedback commands implemented through Python, enables intelligent and adaptive sample processing, enhancing productivity and accuracy. Furthermore, the straightforward integration of SIAsoft with external software systems facilitates compatibility and collaboration with existing laboratory processes, enabling a seamless and integrated laboratory environment.
Altogether, this bioreactor sampling system provides numerous benefits, including flexible and precise sample handling, efficient clarification, real-time monitoring, long-term usability, and seamless integration. These features collectively enhance productivity, reliability, and flexibility in laboratory operations, making the ProSIAmpler system a valuable asset for research and production engineering. See our entire SIA product line to learn more, or reach out to a FIAlab scientist for more information regarding your applications here.
Advantages of SIA
Application Range
SIA can be used in many processes in a on broad range of applications.
On-line Monitoring
On-line monitoring, feedback, and control of your process.
Python Scripting
Easily create custom protocols with Python scripting in SIAsoft.
Automation
Automate sterile sample draw, clarification, purification, titer calculation, normalization, pretreatment, and LCMS injection in one software.
Plastics
Eliminate need for pipette tips and 96-well plates.
Reagent Waste
Little reagent waste and very low consumable costs.
Sample Pretreatment Assays
This automated in-line LC peptide mapping pre-treatment method can be used on any LC unit setup for the multi-attribute monitoring (MAM) of biotherapeutics. The method utilizes a denaturant, reductant, alkylation agent, desalting, and trypsin digestion to prepare a biological sample prior to LCMS analysis.
Peptide Mapping
Peptide Mapping Publication:
This method is for the determination of amino acid species in cell culture media. The method utilizes the Water’s proprietary labeling reagent “AccQ Tag” for derivatizing amino acids prior to HPLC analysis. Intended as a single reagent chemistry, the Amino Acid Analysis package is used for cell culture monitoring.
Amino Acid Monitoring
Amino Acid Publication:
The glycan system automates sample handling to determine stoichiometric relationships between different sugar moieties on monoclonal antibodies (mAbs). This in-line process handles fluid samples by some or all of the following operations: Pro A purification, PNGase enzymatic digestion, fluorescent labeling, and enrichment in porous graphitic carbon (PGC) column.
Glycan Analysis
Glycan Publications: